Analysis of Bacterial Communities in Kusaya Gravy by Denaturing Gradient Gel Electrophoresis of PCR-Amplified Ribosomal DNA Fragments.
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چکیده
منابع مشابه
Analysis of bacterial communities in the rhizosphere of chrysanthemum via denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA as well as DNA fragments coding for 16S rRNA.
The effect of developing chrysanthemum roots on the presence and activity of bacterial populations in the rhizosphere was examined by using culture-independent methods. Nucleic acids were extracted from rhizosphere soil samples associated with the bases of roots or root tips of plants harvested at different stages of development. PCR and reverse transcriptase (RT) PCR were used to amplify 16S r...
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Denaturing gradient gel electrophoresis (DGGE) of a 16S ribosomal DNA PCR product was used to differentiate 32 mycoplasma species of veterinary significance. Twenty-seven (85%) species could be differentiated by DGGE. This method could enable the rapid identification of many mycoplasma species for which there is no specific PCR available and which are currently identified by using culture and s...
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Bacteria play an important role in the initiation and progression of diseases (not only in disease but affect health status of individual). Populations of digestive microflora in chicken gut change with age and also affected by diet, stressors, and performance enhancers as well as in disease conditions. The aim of the pre-sent study was to know bacterial makeup in chicken gut, using denaturing ...
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The bacterial community in partially purified water, which is prepared by ion exchange from tap water and is used in pharmaceutical manufacturing processes, was analyzed by denaturing gradient gel electrophoresis (DGGE). 16S ribosomal DNA fragments, including V6, -7, and -8 regions, were amplified with universal primers and analyzed by DGGE. The bacterial diversity in purified water determined ...
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Nucleic acid-based techniques allow the exploration of microbial communities in the environments such as the rhizosphere. Azospirillum brasilense, a plant growth promoting rhizobacterium (PGPR), causes morphological changes in the plant root system. These changes in root physiology may indirectly affect the microbial diversity of the rhizosphere. In this study, the changes in the rhizobacterial...
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ژورنال
عنوان ژورنال: Japanese Journal of Food Microbiology
سال: 2002
ISSN: 1340-8267,1882-5982
DOI: 10.5803/jsfm.19.179